 หัวเรื่อง:การคัดเลือกแคลลัสของข้าวพันธุ์ขาวดอกมะลิ 105 ให้ทนทานต่อสารกำจัดวัชพืชไกลฟอเสท ผู้เขียน: Suriyan Cha-um, Srisom Surawattananon, Kramolpun Namwongprom,  นายเฉลิมชัย วงศ์วัฒนะ, อาจารย์ สื่อสิ่งพิมพ์:pdf AbstractCalli of KDML105 were selected for glyphosate-tolerance on solidified 0.25% Phytagel?-solidified MS (Murashige and Skoog, 1962) selection medium (MS salts and vitamins, 1 mgl-1 kinetin, and 10-4 M glyphosate) at 25?2 ?C, 70?5 ?mol m-2s-1 photosynthetic photon flux density (PPFD), and 16 hd-1 photoperiod. Calli fresh weight were decreased in selection medium more than in glyphosate-free medium. The selected calli at 4 and 8 weeks (S4 and S8) could be regenerated on the glyphosate-free medium. Plantlets were propagated by tissue culture technique and treated with 0, 10-5, 5?10-5 and 10-4 M glyphosate in vitro. The height of S4 and S8 plantlets was decreased with increasing glyphosate concentrations in culture media. The number of tillers and number of roots were increased in culture media supplemented with 10-5 M glyphosate. The S8 plantlets could survived on the culture supplemented with 5?10-5 M glyphosate. Increasing the height of plantlets indicated high survival percentage under glyphosate-stressed condition. Tolerant plants were transferred to pots and treated with 0, 250, 500, 1000 and 2000 mgl-1 glyphosate. The growth of plants was inhibited and phytotoxicity was observed when treated with high glyphosate concentrations. Increasing glyphosate concentrations resulted in lesser toxicity in S8 plantlets than in S4 plantlets. |
หัวเรื่อง:การเพาะเลี้ยงเนื้อเยื่อข้าวพันธุ์นางมลเอส-4 ผู้เขียน:Suriyun Cha-um, Srisom Surawattananon, Kramolpun Namwongprom, นายเฉลิมชัย วงศ์วัฒนะ, อาจารย์ สื่อสิ่งพิมพ์:pdf AbstractCalli were induced from rice embryo ( Oryza sativa L. cv. Nang Mol S 4 ) on Murashige and Skoog ( MS ) supplemented with 2.0 mg/l 2,4-dichlorophenoxyacetic acid ( 2,4-D) in the dark condition. Proliferated calli were obtained on the MS supplemented with 2.0 mg/l 2,4-D in the same condition, Calli were regenerated on MS supplemented with 1.0 mg/l kinetin and 1.0 g/l casein hydrolysate under light condition. Roots were induced from shoots on the MS-free hormone or supplemented with indolebutyric acid ( IBA ) 0, 0.5, 1.0 or 2.0 mg/l). Servival of rice plants were 100 percentage after transplants under natural condition. |